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custom made spotted microarray  (Full Moon BioSystems)

 
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    Full Moon BioSystems custom made spotted microarray
    Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
    Custom Made Spotted Microarray, supplied by Full Moon BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/custom made spotted microarray/product/Full Moon BioSystems
    Average 90 stars, based on 1 article reviews
    custom made spotted microarray - by Bioz Stars, 2026-06
    90/100 stars

    Images

    1) Product Images from "3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models"

    Article Title: 3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models

    Journal: BMC Pharmacology & Toxicology

    doi: 10.1186/2050-6511-14-11

    Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a microarray scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
    Figure Legend Snippet: Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a microarray scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).

    Techniques Used: Immunofluorescence, Concentration Assay, Methylation, Microarray, Binding Assay, Microscopy



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    custom made spotted microarray  (Full Moon BioSystems)
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    Full Moon BioSystems custom made spotted microarray
    Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a <t>microarray</t> scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).
    Custom Made Spotted Microarray, supplied by Full Moon BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a microarray scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).

    Journal: BMC Pharmacology & Toxicology

    Article Title: 3-D DNA methylation phenotypes correlate with cytotoxicity levels in prostate and liver cancer cell models

    doi: 10.1186/2050-6511-14-11

    Figure Lengend Snippet: Specificity and sensitivity of used anti-methylcytosine antibody. ( A ) The antibody properties were assessed by an indirect immunofluorescence assay, in which the monoclonal anti-MeC antibody for this study — used at the concentration of 1 μg/ml in combination with a secondary antibody (Cy3-conjugated anti-mouse IgG1 at 5 μg/ml), i.e. at the same concentrations as in the cellular assay — was hybridized to a spotted array with two types of short 24-mer oligonucleotides immobilized onto a glass slide: C-oligo that included two CG dinucleotides and its methylated counterpart, the MeC-oligo printed at various dilutions that correlate with different approximate CpG copy numbers (10 10 –10 4 ) . Each DNA probe was spotted as octuple. The specific antibody, detected with a microarray scanner at 5 microns resolution, shows best signal-to-noise (background and non-specific binding to unmethylated C-oligo) ratio at a copy number of 10 10 . The signal (false-colored in green) decreases in a CpG copy number-dependent manner. ( B ) Similar average intensities were obtained, when a sub-area (magenta box in Figure A) of the same array was subjected to confocal scanning microscopy at 200 nm horizontal resolution. The line scan (magenta) shows the more detailed intensity profile across the four different types of spots and the intermediate gaps (coated glass slide/background).

    Article Snippet: Antibody testing was performed by an immunofluorescence assay utilizing a custom made spotted microarray (Full Moon Biosystems) comprising multiple copies of two synthesized 24-mer oligonucleotide probes that were immobilized onto glass microscopic slides: 5 ′ -TCGTTTTTTTTTTTTTTTTTTCGT-3 ′ (C-oligo) (MWG Biotech), and its counterpart 5 ′ -T me CGTTTTTTTTTTTTTTTTTT me CGT-3 ′ (MeC-oligo) (Biopolymers-Thermo Scientific), in which the two cytosine molecules were replaced by methylcytosine.

    Techniques: Immunofluorescence, Concentration Assay, Methylation, Microarray, Binding Assay, Microscopy